A DNA-Assisted Binding Assay for Weak Protein–Protein Interactions
نویسندگان
چکیده
منابع مشابه
A DNA-assisted binding assay for weak protein-protein interactions.
We describe a new method used for quantitating weak interactions between proteins in which the weak interaction is "assisted" by a known DNA-DNA interaction. Oligonucleotides, which are conjugated to proteins of interest, contain short complementary DNA sequences that provide additional binding energy for protein-protein interactions. A stretch of unpaired bases links the protein to the hybridi...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2009
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2009.09.064